LightSheet
From single molecules to whole organisms, 3i LightSheet systems enable live cell imaging beyond the limitations of traditional confocal modalities
Axially Swept Light Sheet Microscopy System for Imaging Cleared Specimens
Brain
Thy1-GFP mouse brain cleared using the PEGASOS technique. The entire brain was captured at 1µm x 1µm x 4µm resolution using the Hamamatsu Fusion BT sCMOS camera in 1.6 hours and rendered in Aivia.
DROSOPHILA
Dual-view stage scan capture of a Drosophila wing disc showing Viking (magenta) and membrane (green). Courtesy of Dr. Yanlan Mao and Dr. Rob Tedley, University College London Laboratory for Molecular Cell Biology (LMCB).
MEMBRANE DYNAMICS
HeLa cells transfected with GFP-Lifeact (488nm) to visualize actin dynamics and stained with DiL (561nm red)(Thermo) to visualize membrane dynamics. Courtesy of James Springfield, Institute for Molecular Bioscience (IMB) at the University of Queensland
Widefield, Confocal, Multiphoton, and TIRF
Bespoke multimodal microscope systems with any combination of multiphoton, spinning disk confocal, structured illumination, spinning TIRF, photomanipulation, computer-generated holography, and more
CANCER RESEARCH
Prostate tissue section labeled for nuclei (blue) and blood vessels (orange).
THROMBOINFLAMMATION
Neutrophils and platelets in the inflamed cremasteric venules of a WT mouse, 3h after local injection of TNFa. Courtesy of Dr. Vinatha Sreeramkumar & Dr. Andrés Hidalgo (Centro Nacional de Investigaciones Cardiovasculares).
DROSOPHILA NEURONS
T4T5 neurons in the Drosophila visual system responding to various directions of visual motion. Courtesy of Dr. Ben Hardcastle, Frye Lab, University of California Los Angeles.
Top Image Credits (Left to Right): Isabella Osterlund & Dr. Felix Ruhnow at University of Copenhagen, Jenna Sternberg at Institut du Cerveau et de la Moelle Epiniere, Alain Chedotal at Vision Institute, Laura Desban, Andrew Pendergast & Claire Wyart at Institut du Cerveau et de la Moelle Epiniere, Hella Broughton at 3i UK, Andrew Barazia at 3i Denver